ABSTRACT
To study the effect of ketoconazole induced liver damage, compare with control group and correlate with previous studies. Experimental study conducted during year 2005 in the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre,Karachi. Forty adult male albino rates were used for this study. Group-A served as control animals, received injection of normal saline in dose of 0.05 ml/100 gm of body weight inraperitoneally daily for 03, 07, 05 and 30 days. Group-B received injection of ketoconazole 40 mg/kg of body weigh intraperitoneally daily for 03, 07, 15 and 30 days of treatment. Animals were sacrificed after completion of treatment under ether anaesthesia. Liver were removed, fixed in 10% and alcoholic formalin for 24-48 hours. They were dehydrated in ascending strength of alcohol and paraffin tissue blocks were made 5 microm thick section were stained with HandE for general morphology and micrometry and the result were compared with control. Analyzed satistically with student 't' test and correlate with previous studies. Ketoconazole treated animals showed distortion of hepatic architecture increase size of hepatocytes, decrease nuclear diameter and necrosis of hepatocytes within hepatic lobule as compared to control group-A animals. It was sconcluded from this study that ketoconazole induced injury is dose and duration of therapy dependent and due to its cost effective frequent use needs further research in humans